From HoNWiki

On pickup, runes temporarily apply varied effects:

Double Damage Effect +100% base damage Duration 2:00	Haste Effect Grants unit max movespeed (522) Duration 30s	Illusion Effect Spawns two images that take 300% damage and deal 35% damage Duration Images last 75 seconds
Invisibility Effect Grants invisibility with a 0.1s fade time Duration 30s or until invisibility is broken	Regeneration Effect 6% Health regeneration per second 10% Mana regeneration per second Duration 30s or until Health and Mana are full, or until unit receives any damage

Runes can also be picked up with an empty bottle. Once bottled, a rune may be used at any time or stored up to 2:00, at which point it is automatically used. When a rune is used from a bottle, the bottle is completely refilled.

[edit] Rune spawning

Runes spawn at one of two locations on the map, every two minutes, starting at 0:00.

Screenshots to show rune locations on the maps

	Forests of Caldavar	Watchtower	Darkwood Vale
Top/Left Rune
Bottom/Right Rune

[edit] See Also

Using Runes